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L-赖氨酸产生菌FH128菌株的研究(Ⅰ)FH128菌株的选育及摇瓶发酵

Studies on L Lysine Producing Strain FH128 (Ⅰ) The Screening of L Lysine Producing Strain FH128 and Shake Flask Culture

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【作者】 富英华苏令呜项泽宇

【Author】 Fu Yinghua, Su Lingming, Xiang Zheyu (Shanghai Institute of Industrial Microbiology,Shanghai 200233)

【机构】 上海市工业微生物研究所

【摘要】 以赖氨酸产生菌A111(HS-、AECr)为出发株,经化学诱变剂MNNG(N-甲基-N′-硝基-N-亚硝基胍)及单氟醋酸处理获得单氟醋酸抗性突变株F79,摇瓶发酵产L-赖氨酸盐酸盐7.0%~7.5%,对糖转化率38%~40%,分别比A111株提高约25%及20%。然后,再以F79菌为亲株经MNNG及噻唑丙氨酸处理获得噻唑丙氨酸抗性突变株FH128,在适宜的培养条件下,摇瓶发酵产L-赖氨酸盐酸盐8.5%~9.5%,最高产酸率11%,对糖转化率45%~50%。在16L自控发酵罐发酵,产L-赖氨酸盐酸盐12%~14%,对糖转化率40%~45%;在20~100m3发酵罐发酵,产酸率为8.5%~9.5%,对糖转化率40%~42%,提取总收率80%~85%,成品(饲料级L-赖氨酸盐酸盐)质量符合国家标准(GB8245-87)。FH128菌株遗传性能稳定,营养要求粗放,工艺较简单,便于工业化,二年前已应用于工业生产。

【Abstract】 A resistant mutant of F79,the lysine producer, was derived from the parent strain A111(HS -、AEC r) by mutation with MNNG(N methyl N′ nitro N nitrosoguanidine) and MF(Monfluoroactic acid).The production of L lysine·HCl of the mutant in flask was 7.0% ̄7.5%,the conversion rate of glucose to L lysine was 38% ̄40%.The TA(α Thiazol DL Alanine) resistant strain FH128 was derived from the parent strain F79 by mutation with MNNG and TA.Under optimal culture coditions,the production of L lysine·HCl was 8.5% ̄ 9.5%; the maxium production of L lysine·HCl was 11%,the conversion rate of glucose was 45% ̄50% in shake flask.In 16 L fermenter,the production of L lysione·HCl was 12% ̄14%,the coversion rate was 40% ̄45%.In 20 m 3 ̄100 m 3 fermenter,the production of L lysine·HCl was 8.5% ̄9.5%,the conversion rate was 40% ̄42%.

【关键词】 调节突变株发酵L-赖氨酸
【Key words】 Regulatory mutantFermentationL lysine
【基金】 国家“八五”科技攻关项目
  • 【文献出处】 工业微生物 ,INDUSTRIAL MICROBIOLOGY , 编辑部邮箱 ,1998年04期
  • 【分类号】TQ922.3
  • 【被引频次】1
  • 【下载频次】114
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