【摘要】 对溶血离心血培养法分离伤寒、副伤寒沙门氏菌进行了系列的实验研究。筛选出了0．04mg／ml的理想溶血剂使用浓度。检出阳性最小标本量和最小细菌检出量比较结果表明，溶血离心培养法敏感度优于常规培养法。将溶血离心培养法初步应用于临床及伤寒流行现场并与常规法配对试验，细菌分离阳性率分别为35．12％和26．79％，前者明显高于后者（P＜0．05）。溶血离心血培养法可排除血标本中各种抑菌物质的影响，从而提高细菌的检出率，并能明显缩短分离、鉴定周期，对阳性标本还能进行菌落计数。更多还原

【Abstract】 A series of research of L - C have been done in order to study reliability and availability of separating Typehoid Paratyphoid Salmonella. Our studies have showed that the ideal concentration of haemolysis substance used in L-c is 0. 04 mg/ml, and L-C is more sensitive than traditional culture method in detecting minimal amount of sample and minimal bacteria amount of sample. The bacteria separating rate (35. 12% ) in L - C is higher than that in traditional culture method (26. 79 % ) (P < 0. 05). and the bacteria separating period can be shortened.更多还原