【摘要】 在液相色谱梯度淋洗过程中，由于柱外过程和流动相在柱内的输运过程中的延迟作用和扩散的影响，使得溶剂浓度梯度变化时，在柱内特定位置的浓度随时间的变化符合相应的分布规律．本文从理论和实验两个方面对这一规律加以探讨，并讨论了仪器条件对该分布规律的影响．更多还原

【Abstract】 In the gradient elution of HPLC, the delay and dispersion of mobile phase during the transmission of mobile phase in column, and the out column effect result in that, there is a gradient distribution in the variation of mobile phase content with time at any location in column, and it obeys a distribution law. In this paper, the law is discussed theoretically and experimentally, and the effect on the law of instrumental condition and different solvents is also discussed.更多还原