【摘要】 提出柱前衍生非水毛细管电泳分离金属离子的方法．应用新合成的２－（６－甲基－２－苯并噻唑偶氮）－５－二乙胺基酚作为柱前衍生试剂，乙醇和Ｎ，Ｎ－二甲基甲酰胺为非水溶剂，在６ｍｉｎ内实现了钴、镍和铜的分离，灵敏度高，检测限分别为９．１８×１０－８ｍｏｌ／Ｌ、２．７９×１０－７ｍｏｌ／Ｌ和４．４７×１０－７ｍｏｌ／Ｌ．考察了柱前衍生条件、非水溶剂配比以及分离参数对分离的影响．更多还原

【Abstract】 Separation of cobalt, nickel and copper elements as their 2 (6 methyl 2 benzothiazolyazo) 5 dimethylaminophenol complexes by capillary electrophoresis in nonaqueous medium was first presented. Ethanol and N,N dimethylformamide(DMF) were evaluated for the effect of nonaqueous solvents on electroosmotic flow. The influence of the pre column derivitization conditions, some parameters such as choice and selection of organic solvents, concentration ratio in mixed nonaqueous medium, applied voltage on the separation were also investigated. Under the optimum conditions(buffer: 40 mmol/L, pH 5.0 NaAc HAc in ethanol(50%) and DMF(50%) mixture, 30 kV, 575 nm as detection wavelength), the three ions were separated in 6 min successfully, and detection limits of 9.18×10 -8 mol/L, 2.79×10 -7 mol/L and 4.47×10 -7 mol/L for cobalt, nickel and copper were achieved, respectively. The theoretical plate number reached up to 3.2×10 5/m.更多还原