【摘要】 在电位型尿素酶电极的组装过程中，要求尽量不改变尿素酶的构型和构象，使其在酶膜中保持其自然状态，从而可获得较高的酶活力．用ｐＨ玻璃电极作原电极，将尿素酶固定在其表面，常用的有戊二醛交联法［１］和各种聚合物膜法［２～４］．本文利用６０％季铵化的聚（４－乙...更多还原

【Abstract】 Based on electrostatic interaction between 60% quaternized poly(4 vinyl)pyridine and urease molecules, which can be immobilized on the surface of pH glassy electrode. By means of this technique, the enzyme electrode for urea was prepared. In experiments, we discussed in detail effects of kinds, concentration and pH of media on response of the electrode. In 0.025 mol/L phosphate buffer solution(pH 6.8),the linearity range is between 1×10 -4 and 6×10 -2 mol/L and the detection limit is 1×10 -4 mol/L. The electrode has been applied to the determination of urea in standard serum and recovery is between 92.9% and 107 0%.更多还原