节点文献
地高辛标记DNA探针应用于凝胶转移电泳
The Digoxigenin Labeled DNA Probe Is Applied to Gel Shift Electrophoresis
【摘要】 半抗原DIG-ddUTP与DNA连接,以此作为探针可以与其相识别的蛋白质相结合,再用带有碱性磷酸的抗体处理,形成的复合物可作用于底物CSPD,使其产生萤光,而被x光片记录下来。
【Abstract】 Hapten DIG- ddUTP is combined to DNA by terminal transferase. The DNA probe associates with its specific protein. The DNA-Protein is treated with anti-DIG antibody, Fab fragments coupled to alkaline phosphatase, and thereby forms a complex. The complex can catalyze its substrate CSPD to produce chemilu-minescent signals which are recorded by x-ray film.
- 【文献出处】 阜阳师范学院学报(自然科学版) ,Journal of Fuyang Teachers College Natural Sciences , 编辑部邮箱 ,1998年04期
- 【分类号】Q503
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