【摘要】 通过电化学聚合法制备了聚Ｎ，Ｎ－双水杨醛乙二胺合钴［ｐｏｌｙＣｏ（Ｓａｌｅｎ）］修饰超微电极，研究了该修饰电极的电催化性质及其在一氧化氮（ＮＯ）测定中的应用．实验结果表明，ｐｏｌｙＣｏ（Ｓａｌｅｎ）修饰超微电极对ＮＯ的测定有高的灵敏度，ＮＯ的浓度在２．０×１０－８～２．８×１０－６ｍｏｌ／Ｌ范围内，电流与浓度呈良好的线性关系，线性相关系数为０．９９９８，检出限为１．０×１０－８ｍｏｌ／Ｌ；该电极进一步修饰Ｎａｆｉｏｎ后，生物体中常见的物质如抗坏血酸、儿茶酚胺类神经递质的代谢物、ＮＯ的氧化产物ＮＯ－２等不干扰测定．本传感器可以满足ＮＯ在体分析的需要．更多还原

【Abstract】 The preparation of the ultramicroelectrode modified with an electropolymerized film of ethylenebis(salicyldiminate) cobalt [polyCo(Salen)],its activity for catalytic oxidation of nitric oxide (NO) and its application in NO determination were described in this paper. It is found that that polyCo(Salen) modified ultramicroelectrode shows a high sensitivity to NO determination and that NO concentration was linear with the oxidation current in the range of 2.0×10 -8 ～2.8×10 -6 mol/L with a detection limit of 1.0×10 -8 mol/L,linear coefficient is 0.9998 . The ultramicroelectrode could be free from the interference from some coexisting biological substances in biological fluid,such as ascorbate,nitrite and the matebolites of monoamines . The ultramicroelectrode should be promising in in vivo determination of NO .更多还原