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乙基紫标记分光光度法测定脱氧核糖核酸

Spectrophotometric Determination of Deoxyribonucleic Acid Labeling With Ethyl Violet

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【作者】 李天剑沈含熙罗云敬

【Author】 Li Tianjian, Shen Hanxi, Luo Yunjing (Department of Chemistry, Nankai University, Tianjin 300071)

【机构】 南开大学化学系!天津 300071

【摘要】 研究了阳离子染料乙基紫与脱氧核糖核酸(DNA)的结合反应。在pH=6.4~7.4的条件下,乙基紫在595nm处有最大吸收峰,当加入DNA后,乙基紫的吸收峰显著下降,而在507nm处出现新的吸收峰。以乙基紫为标记物,根据其595 nm吸收峰下降的程度,可用于DNA的定量测定。DNA的线性响应范围为0~1.08 × 10-5 mol/L;表观摩尔吸光系数ε595=7.24 ×104 L·mol-1·cm-1。方法具有较高的灵敏度和选择性。用于合成试样分析,结果满意。

【Abstract】 The binding reaction of DNA (calf thymus deoxyribonucleic acid) with dye ethyl violet has been studied. In the medium of pH range 6 .4 - 7. 4, ethyl violet showed a decrease in absorption at the wavelength of its maximal absorption peak (595 um) on binding to DNA, and at the same time a new peak of the binding product appeared at 507 um. A novel method for the determination of DNA at 595 um using ethyl violet as a labeling agent was developed. The method is of high sensitivity as well as selectivity, and has been used to determine DNA in synthetic Samples with satisfactory results.

【基金】 国家自然科学基金!(29675009)
  • 【文献出处】 分析化学 ,Chinese Journal of Analytieal Chemistry , 编辑部邮箱 ,1998年11期
  • 【分类号】O657.3
  • 【被引频次】71
  • 【下载频次】162
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