【摘要】 铂丝（Φ０．３ｍｍ）经铂化处理后，形成疏松、粗糙的表面，将四硫富瓦烯·四氰基奎诺 二甲烷（ＩＴＦ·ＴＣＮＱ）导电有机盐嵌入到铂化的铂黑微粒中。将３－羰基丁酸吡咯与吡咯按适当 的比例在其表面用电化学聚合方法，将葡萄糖氧化酶（ＧＯＤ）固定于聚合形成的杂聚膜中制成 微酶电极。该做酶电极测定时几乎不受氧分压影响，灵敏度高（４．８ μＡ／ｍｍｏｌ·Ｌ－１），响应快 （＜１０ｓ）。由于所需极化电位低（５０ ｍＶ极化电压下已接近最大的响应电流）以及杂聚膜的作 用，抗坏血酸、尿酸、乙酸氨基酚等常见干扰物对电极测定没有任何影响。测试初期电极灵敏 度有所下降，之后四个月电极灵敏度基本保持不变。更多还原

【Abstract】 Conductive organic salt tetrathiafulvalene tetracyanoquinodimethane(TTF. TCNQ) and platinum particle complex were formed by dipping platinized platinum wire into TTF and TCNQ solution sequencely, glucose oxidase was immobilized on the microelectrode surface by electropoloymerization of a solution of pyrrole and 4 - (3 - pyrrolyl) -4 - oxobutiric ac id to form a heteropolypyrrole / glucose oxidase film. The micro-enzyme electrode showed high sensitivity (4. 8 μA/mol’ L-1) at low applied potential (50 mV), short response time(10 s), long stability, oxygen and interference free.更多还原