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甘油三酯微生物传感器的研制

Studies On Microbial Sensor for Triglyceride

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【作者】 李贵荣王永生占利生吕昌银吴移谋刘传湘

【Author】 Li Guirong; Wang Yongsheng; Zhan Lisheng; Lu Changyin, Wu Yimou, Liu Chuanxiang (Hengyang Medical College, Hengyang, 421001)

【机构】 衡阳医学院化学教研室!衡阳421001

【摘要】 以白地霉菌为甘油三酯水解反应的酶源,采用pH玻璃电极为基础电极研制成甘油三酯微生物传感器。对细菌的筛选和培养条件、传感器制作方法和传感器的响应性能进行了研究。在28℃时,选择磷酸盐缓冲溶液(pH7.5)为测量介质,测得所制传感器对甘油三酯的响应线性范围为:3.94× 10-6~ 1.91 × 10-3 mol/L,响应斜率为:37.4 mV/dec;响应时间为:5~15 min。使用寿命可达28 d。

【Abstract】 A microbial sensor for the determination of triglyceride was prepared in this paper. Geotri chum candidum was used as the biocatalyst and pH glass electrode was used as the base electrode. The effects of the culture medicum, immobilized methods of the microorganism and the response characteristics of the sensor were studied. The response of the sensor has a linear range between 3.94 × 10-6 - 1 .91 × 10-3 mol/L triglyceride with a slope of 37 .4 mV/dec in the pH 7.5 phosphate buffer solution at 28 ℃. The response the is 5 - 15 min. The sensor is stable for28 days.

【基金】 湖南省教委资助课题
  • 【文献出处】 分析化学 ,Chinese Journal of Analytieal Chemistry , 编辑部邮箱 ,1998年07期
  • 【分类号】TP212
  • 【被引频次】7
  • 【下载频次】89
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