【摘要】 目的为了解决二氯苯醚菊酯中毒检验的问题及进一步开展研究工作，建立多种生物材料中二氯苯醚菊酯高效液相色谱分析方法。方法选用shim—pack，CLC—ODS（5μm）作色谱柱，甲醇：水：冰醋酸（90：10：1）为流动相，检测波长为274mm。用空白添加试验系统地考察提取，净化方法及高效液相色谱分析条件。用日本大耳兔进行急性染毒，取材对所建方法进行验证。结果采用所建方法，空白组织添加回收率在0．2～20μg／g的范围内均大于85％；工作曲线线性良好（r＝0．9998）；以信噪比≥3计，最低检测限为10ng／g组织；方法重现性良好（CV＜4．5％，n=3）；染毒兔各组织中的内源性杂质均不干扰二氯苯醚菊酯的检测。结论所建方法适用于检测多种生物材料中的二氯苯醚菊酯，方法灵敏、准确，可望用于二氯苯醚菊酯的中毒检验、代谢动力学及分析毒理学的研究。更多还原

【Abstract】 Objective To establish a RPHPLC method for determining permethrin in various biological mainal. Methods Analytical column was shimpack CLC-ODS (5μm, 150 × 6. 0 mm, I. D), mobile phase was methanol-water-acetic acid solution (90: 10: 1 ) and permethrin was detected at 274 urn. The Methods of extraction, purification and detection conditions were comparative investigated using blank animal tissue and permethrin -treated animal tissue. Results The clean-up recovery of permethrin in the rabbits’ liver was more than 85% within the range of 0. 2-20 μg/g permethrin level. The calibration curves were linear (r= 0. 9998). The detection limit was 10ng/g (S/N≥3). The RSD were no more than 4. 5% (n=3). No interfere by endogenous compounds was observed in study results using this method. Conclusion RPHPLE method shows an excellent recovery, high sensitivity and good precision. Thus, it is suitable for investigation of the permethrin poisoning in various biological material, and its pharmacokinetic and analytical toxicology study.更多还原