【摘要】 从霉变的菜籽粕中筛选出具有产生植酸酶、降解植酸能力的青霉菌株P7。在液态发酵条件下，48h植酸降解率达96％。采用DEAE－52柱层析技术部分纯化植酸酶，对其生物学特征的研究表明：该植酸酶的最适反应温度为60℃，最适反应酸碱度为pH5.0，测得酶的米氏常数为0．25mmol／L。更多还原

【Abstract】 A strain of Penicillium sp. was obtained directly from rapeseed meal with selecting culture medium. This strain is able to produce phytase which degrades phytic acid. In liquid medium,96% of the phytic acid was hydrolysed at 30℃ in 48 h by the organism. The production of phytase by the strain was affected by pH of the medium,fermentation time,fermentation temperature,composition of the medium and the aeration condition. The phytase was partially purified from the culture broth of Penicillium sp. P7 by using DEAE-52 chromatography and the characteristics of the phytase was studied. The enzyme showed an optimum pH of 5.0 and an optimum temperature of 60℃. The Km value of the enzyme was 0. 25 mmol/L.更多还原