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苏云金杆菌对落叶松毛虫毒力测定
Isolation and Determination of A Plant Pathogen of Dendrolimus superans
【摘要】 1993年在大兴安岭林区,从落叶松毛虫(DendrolimussuperansButler)自然死亡虫体内分离到一株病原细菌,经过分离、纯化、培养,这是一株能产生芽孢和伴胞晶体的苏云金杆菌(Bacilusthuringiensis),定名为L-93菌株。通过生理生化反应试验和血清学鉴定,确定该菌的血清型为H3a3b,但生化反应与库斯塔克变种略有不同,其特点是尿酶反应为阴性。对落叶松毛虫幼虫回接试验,5d校正死亡率达972%。该菌株的发现为在寒冷地区开展生物防治森林害虫提供了一个优良菌株。
【Abstract】 A bacteria plant was separated from the natrural dead D superans in the Daxing′anling forest region in 1993 By proving it is the Bacillus thuringiensis which can produce spore and crystal It is named as L-93 bacteria It′s blood serum type is H 3a3b , But it′s biochemistry reaction is different from the known H 3a3b B thuringensis var kurstaki It′s urease reaction is negative The dendrolimus correct death rate for five days is 97 2% It′s a good bacteria plant for the prevention and control of forestry diseases in cold region
【Key words】 Bacillus thuringiensis; Dendrolimus superans; Physiological and biochemistry reaction; Serological text;
- 【文献出处】 东北林业大学学报 ,JOURNAL OF NORTHEAST FORESTRY UNIVERSITY , 编辑部邮箱 ,1998年01期
- 【分类号】Q93-331
- 【被引频次】9
- 【下载频次】84