【摘要】 <正> 小肠结肠耶氏菌能引起人类腹泻、关节炎、阑尾炎和败血症等疾病。常见的致病性耶氏菌为0:3、0:9血清型,而0:22血清型被视为非致病型。该文用聚合酶链反应、DNA序列分析、随机扩增多态性DNA及刚果红致病性试验对其致病性进行探讨。 1,材料和方法 1.1菌株:购于中国菌种保存中心,0:22血清型3株,菌号52229;0:3血清型3株,菌号52301;0:9更多还原

【Abstract】 Yersinia enterocolitica can cause diarrhea dis-eases, arthritis, appendicitis, and sepsis in human be-ing. The major pathogenic serotype is 0:3, 0:9 bio-type, while 0:22 has been regarded as nonpathogenicbiotype. The methods Polymerase chain reaction(PCR), DNA sequence analysis, randomly amplifiedpolymorphic DNA (RAPD) and Congo red test wereused to study the pathology of 0:22 serotype for thebenefit of its provention. 1 Bacteria strains: 3 strains 0:22, No. 52229, 3strains 0:3, No. 52301, 2 strains 0:9, No. 52302,obtained from National Institute for the control ofPharmaceutical and Biological Products, Beijing. 2 PCR: Primers was designed from ail gene seg-ment and PCR amplified as the report. 3 DNA sequence analysis: the products of PCRwere pured with Clontech Co. USA PCR pure kit.The DNA segments were analyzed with PE Co. USAGenetic Analyzer. 4 Congo red test: finished according to the re-port. Result 3 strains 0:22, 3 strains 0:3 and 2 strains 0:9, were amplified by PCR, produced 359bp DNA seg-ments. Analysis of DNA sequence: 359bp PCR productsof 0: 22 strain and 0: 3 were in general same. RAPD: 0: 22, 0: 3 and 0: 9 strains fingerprintswere different from each other, while they had a samesize major band. Congo red test: a few colon were positive withturning red. Discussion The major pathogenic serotype has been 0: 3 and0: 9 strains, while 0: 22 has been regarded non-pathogenic. This result showed that 0: 22 strains hadpathogenic ail gene as 0: 3, which were confirmed byanalysis of DNA, and same colon were positive withCongo red test and were consistent with the report.RAPD fingerprints showed that there were similar in-heritance characteristics. The results showed thatyersinia enteroclitica 0: 22 serotype not only has thepathogenic ail gene but also can express in some cases.It was reported that 0: 22 strain was obtained fromsewage. Thus, we should paid attention to 0: 22serotype.更多还原