【摘要】 目的：研究ＡｒｇＧｌｙＡｓｐＳｅｒ（ＲＧＤＳ）肽对血小板聚集和血小板膜ＧＰⅡｂ／Ⅲａ反磷酸化的影响。方法：免疫沉淀提取血小板膜ＧＰⅡｂ／Ⅲａ，反磷酸化测定。结果：５０μｍｏｌ·Ｌ－１ＡＤＰ引起血小板聚集时，其ＧＰⅡｂ／Ⅲａ反磷酸化明显降低。而应用１００和２００μｍｏｌ·Ｌ－１ＲＧＤＳ肽与ＡＤＰ共同孵育，呈浓度依赖抑制血小板聚集的同时，可逆转ＡＤＰ引起的蛋白反磷酸化改变（Ｐ＜０．０１），其抑制血小板聚集和ＧＰⅡｂ／Ⅲａ反磷酸化呈明显的负相关（ｒ＝－０．７６，Ｐ＜０．０１）。结论：ＲＧＤＳ肽抑制血小板聚集是通过抑制血小板膜ＧＰⅡｂ／Ⅲａ磷酸化所致更多还原

【Abstract】 Objective: To study the effect of Arg Gly Asp Ser(RGDS) peptide on platelet aggregation and back phosphorylation of platelet glycoprotein Ⅱb/Ⅲa during platelet activation. Methods: Immunoprecipitation extract of platelet membrane glycoprotein Ⅱb/Ⅲa, and back phosphorylation. Results: 50 μmol·L -1 ADP, while inducing platelet aggregation, obviously decreased back phosphorylation of platelet glycoprotein Ⅱb/Ⅲa. When platelet and ADP were incubated together with 100, 200 μmol·L -1 RGDS peptide it was found that RGDS peptide markedly inhibited ADP activated platelet aggregation and countered ADP induced phosphorylation of platelet glycoprotein Ⅱb/Ⅲa ( P <0.01) in a concentration dependent manner and the degree of inhibition between platelet aggregation and GP Ⅱb/Ⅲa back phosphorylation were in negative corelation ( r = -0.76 , P <0.01). Conclusion: The inhibition effect of RGDS peptide on platelet aggregation resulted from inhibiting platelet GP Ⅱb/Ⅲa phosphorylation.更多还原