【摘要】 目的：探讨血管紧张素Ⅱ（ＡｎｇⅡ）在肾病水肿及蛋白尿形成中的作用。方法：采用放射免疫分析法及逆转录聚合酶链反应（ＲＴＰＣＲ）技术，检测了阿霉素肾病大鼠血浆和肾组织ＡｎｇⅡ含量及肾血管紧张素ⅡⅠ型受体（ＡＴ１Ｒ）ｍＲＮＡ表达。结果：阿霉素肾病组血浆及肾组织ＡｎｇⅡ水平均高于正常对照，而肾ＡＴ１ＲｍＲＮＡ表达较正常减低。结论：表明阿霉素肾病大鼠存在ＡｎｇⅡ及其受体的异常，ＡｎｇⅡ通过影响肾小球血流动力学参与肾病的水肿及蛋白尿形成。在ＡｎｇⅡ对肾病形成的作用中，局部因素更为重要。更多还原

【Abstract】 Objective: To investigate the role of angiotensin Ⅱ (AngⅡ) and angiotensin Ⅱ receptor (AngⅡ R) in development of edema and proteinuria in nephrotic syndrome. Methods: The expression of renal angiotensin Ⅱ type1 receptor (AT1R) gene in adriamycin nephrotic rats was detected by the methods of radioimmunoassay and reverse transcriptasepolymerase chain reaction (RTPCR). Results: The study showed that the levels of AngⅡ in plasma and renal tissue in experimental group were increased than that in control group. The expression of AT1R mRNA was lower in adriamycin nephrotic rats than that in control. Conclusion: The model of adriamycin nephrotic rats has the abnormalities of AngⅡ and its receptor. AngⅡ may play a role in the development of edema and proteinuria in nephropathy by changes of glomerular blood flow dynamics. The effect of local AngⅡ in renal tissue was more important in the formation of nephrosis.更多还原