节点文献
氮蓝四唑光照法实验操作的改进及效果
THE MODIFICATION OF NBT-ILLUMINATION METHOD AND EFFECT OF THE MODIFIED METHOD
【摘要】 对氮蓝四唑(NBT)-光照法实验操作进行了改进,用改进后的光照法测定了2种自制的SOD模拟物Cu(Gly-Gly)H2O和Cu(Ser)2与超氧阴离于自由基O2-应的动力学参数,其值与其他方法所测一致.对改进效果进行了讨论和评价.结果表明,经改进的光照法在简化操作、提高效益及测量精度方面有很大改观.
【Abstract】 NBT-illumination method is modified. Kinetic parameters for the reactions of analogues of SOD-Cu(Gly-Gly)H2O and Cu(Ser)2 with superoxide anion radical are measured by the modified method, these parameters correspond to the reported values measured by other methods. The effects of modification of NBT-illumination method are discussed and evaluated. The result shows that the modified method simplifies operation, decreases costs and improves precision.
【关键词】 NBT光照法;
SOD模拟物;
反应速率常数;
O2-;
【Key words】 NBT-illumination method; analogues of SOD; rate constant of reaction; superoxied anion radical;
【Key words】 NBT-illumination method; analogues of SOD; rate constant of reaction; superoxied anion radical;
【基金】 国家自然科学基金!29775015
- 【文献出处】 北京师范大学学报(自然科学版) ,JOURNAL OF BEIJING NORMAL UNIVERSITY(NATURAL SCIENCE) , 编辑部邮箱 ,1998年01期
- 【分类号】O626.28
- 【被引频次】64
- 【下载频次】768