【摘要】 将三碘甲状腺原氨酸（T3）与β－D半乳糖昔酶联结，制成T3-酶联结物（T3－E）。用T3－E作为示踪剂建立了游离T3（FT3）酶免疫分析（EIA）。本法灵敏度为0.29pmol／L，批内和批间变异系数分别为7.6％－8.7％和7.8％～14.9％，与二碘甲状腺原氨酸（T2）、反T3（rT3）和甲状腺素（T4）的交叉反应率分别为9.7×10-4、1.1×10-4和3.1×10-5。本法与FT3放射免疫分析（RIA）相关性好（r=0.91，P＜0.01）。用本法测定了36名正常人、16名健康孕妇、15例甲亢患者和15例甲低患者的血清中FT3值，其结果与临床相符。更多还原

【Abstract】 Enzyme immunoassay (EIA) of free triiodothyronine (FT3 ) has been developed,with the conjugation of T3 and galactosidase as an analog and a tracer. The assay has a detection limit of 0. 29 pmol/L. The coefficients of variation within an assay and between assays were 7. 6 %～ 8. 7 % and 7. 8 % ～ 14. 9 %, respectively. The crossreactivities with diiodothyronine (T2 ), reverse T3(rT3 ) and thyroxine (T4 ) were 9. 7 % × 10-4, 1. 1 × 10 -4, and 3.1 × 10-5, respectively.This method and FT3 radioimmunoassay (RIA) were correlated well (r = 0. 91, P < 0. 01 ).Serum levels of FT3 in 36 euthyroid persons, 16 normal pregnant women, 15 hyperthyroid and 15 hypothyroid patients were determined, which were consistent with clinical diagnosis.更多还原