【摘要】 ①目的构建人类巨细胞病毒ＡＤ１６９株即刻早期基因ＵＬ３７×１基因片段的原核表达克隆。②方法通过聚合酶链反应（ＰＣＲ）扩增目的基因，然后用限制性内切酶将目的基因与原核表达载体ｐＢＶ２２０定向连接。③结果获得了重组的原核表达质粒。④结论用限制性内切酶鉴定原核重组表达质粒构建成功更多还原

【Abstract】 Objective To construct the prokaryotic expression plasmid of immediate early gene UL37×1 gene frangment of HCMV strain AD 169 . Methods The target gene was amplified by PCR, and the restricted inserting of the target gene into the prokatyotic expression plasmid pBV220 was finished by restricted endonuclease. Results Recombinant prokaryotic expression plasmid was gained. Conclusion Recombinant prokaryotic expression plasmid was confirmed by restricted endonuclease analysis.更多还原