【摘要】 目的：在体外观察８肽生长抑素（ＳＭＳ）对原代培养大肠癌活细胞数和ＤＮＡ合成的影响，以探讨生长抑素对大肠癌细胞生长的调控作用。方法：对２５例大肠癌根治术病人的新鲜标本进行癌细胞分离和原代培养，采用ＭＴＴ比色分析法检测活癌细胞数（吸光度，Ａ值）；３Ｈ－ＴｄＲ掺入法检测ＤＮＡ合成（脉冲数，ＣＰＭ值）。分ＳＭＳ组、５肽胃泌素（ＰＧ）组、ＳＭＳ＋ＰＧ组及对照组。结果：ＳＭＳ组高、中和低分化腺癌活癌细胞数和ＤＮＡ合成均明显低于对照组（Ｐ均＜０．０５）和ＰＧ组（Ｐ均＜０．０１），ＰＧ组均显著高于对照组（Ｐ均＜０．０１），ＳＭＳ＋ＰＧ组均显著低于对照组（Ｐ＜０．０１～０．０５）及ＰＧ组（Ｐ均＜０．０１）。结论：生长抑素对原代培养大肠癌细胞的生长具有负性调控作用，它不仅具有直接抑制大肠癌细胞生长的作用，而且具有抑制胃泌素对大肠癌细胞的促生长作用，为大肠癌病人应用生长抑素类似物治疗提供实验依据。更多还原

【Abstract】 Objective: To study the regulatory effects of somatostatin (SS) on the growth of large intestine carcinoma cells, the effect of octapeptide somatostatin SMS 201.995 (SMS) on cell viability (absorption value, A value) and DNA synthesis (CPM value) of the primarily cultured large intestine carcinoma cells were evaluated. Methods: The primarily cultured large intestine carcinoma cells were obtained from 25 patienls and divided as follows, SMS group, pentagastrin (PG) group, SMS plus PG and control group, cell viability (A value) was evaluated by using MTT assay, DNA synthesis (CPM value) was done by using 3H-TdR incorporation. Results: In SMS group, A value and PCM value in well, moderately and poorly differentiated carcinoma were lower than those in control (P<0.05) and PG group (P<0.01), Those in PG were those higher than those in control (P<0.01), Those in SMS plus PG were lower than those in control (P<0.01～0.05) and PG group (P<0.01). Conclusion: SMS inhibits not only the growth of primarily cultured large intestine carcinoma cells but also the proliferative effect of gastrin on large intestine carcinoma cells. It is provided an experimental foundation for the therapy of patients with large intestine carcinoma using somatostatin analogues.更多还原