【摘要】 D-半乳糖或臭氧所致模型小鼠灌胃给予灵芝制剂500、250和125mg/kg,测定各组小鼠全血谷胱甘肽过氧化物酶(GSH-Px)活性,血清丙二醛含量以及心、肝、脑组织中脂褐素的含量,以观察灵芝制剂的延缓衰老作用。与 D-半乳糖模型组小鼠比较,灵芝500、250和125mg/kg 可以显著提高全血 GSH-Px 活性(P<0.01);灵芝500mg/kg 能显著降低血清丙二醛含量(P<0.01),也可显著降低心肌脂褐素含量(P<0.01);灵芝500、250mg/kg 可显著降低脑脂褐素的含量(P<0.01),但对于肝脏脂褐素的减少,影响不显著。与臭氧模型组小鼠比较,灵芝500、250me/kg 可以显著提高全血 GSH-Px(活性(P<0.05或 P<0.01),显著降低血清丙二醛含量(P<0.01),但对心、肝、脑脏器脂褐素的减少,影响不显著。结果提示,灵芝可能通过影响代谢过程,清除自由基或抑制自由基反应的酶系,而抑制脂质过氧化,成为灵芝延缓衰老的重要因素。更多还原

【Abstract】 The subaeute aging mice models formed by being injected with D-galactose or being exposed in the environment of ozone （O₃） were given Ganoderma lucidum（GL） 500,250,125 mg/kg （ig）.Then the activities of glutathione peroxidase （GSH-Px） in blood,the contants of malonyldialdehyde （MDA） in serum and lipofuscin of heart,liver and brain tissues of mice were mea- sured to observe the antiaging effects of GL.Compared with the D-galactose-aging mice model group,the activities of blood GSH-Px were significantly increased （P<0.01） after administration of GL 500,250,125 mg/kg,the contents of serum MDA and lipofuscin of heart tissue were significantly decreased after administration of GL 500 mg/kg （P<0.01）,the contents of lipofuscin of brain tissue were significantly decreased after administration of GL 500,250 mg/kg （P< 0.01）.But no significant changes were observed for lipofuscin of liver tissue.Contrast to ozone-aging mice model group,the activities of blood GSH-Px were significantly increased and the contents of serum MDA were signiflcantly decreased after administration of GL 500,250 mg/ kg （P<0.01 or P<0.05）.But no significant changes were observed for lipofuscin of heart liver,brain tissues.The results showed that GL might have antiaging effects through the inhibition of lipid-peroxidation by affecting the metabolic process,elimi- nating free radicals and inhibiting enzyme system of free radical reaction.更多还原