【摘要】 本研究主要观察晶状体中与谷胱甘肽代谢相关的三种酶谷胱甘肽过氧化物酶(GSH-Px),谷胱甘肽还原酶(GSSG-R)、谷胱甘肽硫转移酶(GSH-S)的活性在白内障形成过程中的变化及中药抗氧化方(ACHM)对其影响。结果表明:16日龄亚硒酸钠性白内障的大鼠晶状体内 GSH-Px、GSSG-R、GSH-S 活性升高,与同龄正常大鼠相比差异显著(P<0.01),22日龄时酶活性明显下降,与正常组差异显著(P<0.01),28日龄时亦趋下降。ACHM 可改善硒引起的晶状体中酶活性的改变。16日龄时酶活性无明显升高,22日龄时已接近于正常,28日龄仍趋稳定。ACHM 抗白内障的形成可能与维持晶状体内谷胱甘肽相关酶活性的稳定性有关。更多还原

【Abstract】 The effect of Chinese medicinal herbs anti-cataract herbal mixture （ACHM） on the activity of glutathion peroxidase （GSH- Px）,glutathion reductase （GSSG-R） and glutathion S-transferase （GSH-S） of rat lens were studied during cataractous induction with sodium selenite.It has been found that the enzymes activity of 16 days model rat lens was higher than that of normal （P< 0.01）,in group of Na₂SeO₃（these rats of 16 days old had two injections of Na₂SeO₃）,it still lowered further in rats of 28 days, yet ACHM could keep activity of enzyme at a normal range （P<0.05）.These results suggested that the anti-cataract formation property of ACHM in rats might be related to the maintenance of stability of enzyme activity related to glutathion metabolism in lens.更多还原