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后海穴区CB-HRP轴浆运输追踪的神经元节段性分布及相应运动神经无形态观察

Spinal Segment Distribution of Neural Innervation Related Houhai Acupoint ──Studied by CB-HRP Tracing Method Focused on Observation of the Dendrites of Spinal Motor Neurons

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【作者】 谭会兵; 石葛明; 林嘉友; 刘克; 左志林; 万选才;

【Author】 Tan Huibing; Shi Geming; Lin Jiayou ;Liu Ke; Zuo Zhilin; et al (Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005)

【机构】 中国医学科学院中国协和医科大学基础医学研究所!北京; 100005; 北京农学院; 中国医学科学;

【摘要】 用CB-HRP对大鼠后海穴区进行神经节段性分布的研究。在L6~S木水平标记出该穴相关运动神经元(Mn)的树突形成软膜下树突丛和空管膜下树突丛,两者均为嫌高尔基树突。有些远端树突穿越到对侧灰质。在背角和背连合核(DCN,S1~2)区域内CB-HRP标记的初级跨节感觉纤维和标记的前终末纤维可与送行标记的Mn树突汇聚和重叠。电针或免疫注射动物的Mn,其树突软膜下丛和室管膜下丛减少。结果提示后海穴区的神经支配在脊髓内形成传A-DCN整合-传出模式的神经反射回路,相关Mn(L6~S1)可能参与后海穴位作用。

【Abstract】 The spinal segment distribution of neural innervation related Houhai acupoint in rats hasbeen investigted by using horseradish peroxidase conjugated cholera toxin B subunit (CB-HRP) tracing technique. Spinal motoneurons (Mn) in L6~S1 were labeled retrogradely byCB-HRP, and their features have been characterized: 1) their marginal plexus of the den-drites radially projected to the lateral and/or anterior funiculus and their distal portions.forming subpial dendritic plexus I 2) the medial dendrites ran along the ventral margin of thegray commissure and their distal portions surrounded the central canal, forming asubependymal dendritic plexus. Both of them are Golgi-phobic. Some distal branches of den-drites penetrated to the contralateral gray matter. The dendritic arbors of the labeled mo-toneurons in the group of electro-acupuncture or group of acupoint-inoculation with sheepred blood cells contracted in comparison with that in the normal control group. The primarysensory transganglionic labeled fibers and preterminal fibers revealed by CB-HRP in the dor-sal horn and dorsal commissural nucleus of the spinal cord (L,~S,) converged and overlapedwith the dendrites of retrogradely labeled motoneurons. The results indicated there is a re-flect circuit of afferent-integration-efferent neuro-network in the spinal cord related Houhaiacupoint. The Mns retrogradely labeled by CB-HRP in the L,~S, segments might be in-volved in Houhai acupuncture.

【基金】 国家自然科学基金!39740004和39670379;中国农业部重点基金;中国医学科学院院重点基金
  • 【文献出处】 中国医学科学院学报 ,ACTA ACADEMIAE MEDICINAE SINICAE , 编辑部邮箱 ,1997年05期
  • 【分类号】R322.8
  • 【被引频次】2
  • 【下载频次】62
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