【摘要】 本文研究了无刺根中黄酮化合物的测定方法,并测定了它们的含量。无刺根用热水提取,提取液用聚己内酰胺柱分离,先用30％乙醇洗脱得蛇葡萄素,再用70％乙醇洗脱得杨梅素,用紫外-可见分光光度法测定它们的含量。每百克干样含蛇葡萄素25.2克,相对标准偏差1.7％;杨梅素1.77克,相对标准偏差3.3％。回收率97.0～103％。更多还原

【Abstract】 Ampelopsis cantoniensis (Hook, et Arn. ) Planch, is a Chinese herbal medicine rich in flavonoids. A method to determine flavonoids in the herbal was studied. After the herbal was extracted in hot water, Ampelopsin and myricetin in the extract were separated by polyamide column with eluent of ethanol-water and measured by spectrophotometry. As results, 100g of the dry herbal contains 25. 2g of ampelopsin (RSD=1. 7%, recovery = 97. 0%) and 1. 77g of myricetin (RSD = 3. 3%, recovery=103%).更多还原