【摘要】 该研究采用ELISA法,对肝癌患者的血清检测HCV—Ab,用Nested-PCR法对患者的血清、肝癌组织和癌旁组织检测HCV-RNA和HCV-RNA负链,探讨肝细胞癌中是否存在HCV-RNA及其复制状态。对照组血清检测HCV-Ab、HCV-RNA1/17阳性。肝癌组16/31 HCV-RNA阳性;癌旁组织15例测到HCV-RNA负链,癌组织只有6例测到负链。结论:血清中抗HCV检测不能完全反映肝内是否曾有HCV感染情况。癌组织和癌旁组织均能检出HCV-RNA,说明肝癌组织中感染着丙型肝炎病毒。肝癌组织中确实存在HCV-RNA负链,说明HCV-RNA能在肝组织内不断地自我复制。更多还原

【Abstract】 The presence and replication of HCV-RNA were investigated by examining blood serum,cancer tissue,and para-cancer tissue samples taken from 31 hepatocellular carcinoma patients,as well as blood serum and liver tissue samples taken from a control group consisting of 17 individuals. Hepatitis C and HCV-Ab were studied using ELISA techniques. The positive and negative strands of HCV-RNA were examined using nested PCR. One individual in the control group was found to be HCV-Ab positive in serum,as well as HCV-RNA positive in serum and liver tissue,with both positive and negative stands identified. Of the 31 patients in the liver cancer group, 16 were HCV-RNA positive in the liver. The HCV-RNA positive strand was found in the para-cancer and liver cancer tissue of all 16 HCV positive patients, while the HCV-RNA negative strand was found in the para-cancer tissue of 15 patients, and in the liver cancer tissue of six patients. These results indicate that anti-HCV tests using ELISA in serum can not fully demonstrate HCV infectious conditions,that HCV-RNA exists in liver cancer tissue,and that HCV-RNA is able to continuously replicate in liver tissue.更多还原