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毛花猕猴桃原生质体再生植株根尖染色体数目变异与细胞多核现象

SOMACLONAL VARIATION IN CHROMOSOME NUMBER AND NUCLEI NUMBER OF REGENERATED PLANTS FROM PROTOPLASTS OF ACTIN1DIA ERIANTHA

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【作者】 张远记钱迎倩蔡起贵母锡金魏小萍周云罗

【Author】 Zhang Yuan-ji,Qian Ying-qian,Cai Qi-gui,Mu Xi-jin, Wei Xiao-ping and Zhou Yun-luo(Institute of Botany, Chinese Academy of Sciences, Beijing 100093) (Beijing Plant Cell Bioengineering Laboratory, Beijing 100081)

【机构】 中国科学院植物研究所北京市植物细胞工程实验室中国科学院植物研究所 北京 100093北京 100093北京 100081北京 100093

【摘要】 对18株毛花猕猴桃(Actinidia eriantha Benth.)原生质体再生植株的体细胞染色体数做了观察,其中12株为整倍体:二倍体(2n=58)和四倍体(2n=116)各6株;另外6株为混倍体,其染色体数目变化在59~203之间。还发现原生质体再生植株有丝分裂间期细胞存在多核现象,有多核细胞的共10株,细胞核数目以双核和三核较常见,最多的有7个核。对照植株为2n=2x=58,未发现多核细胞。

【Abstract】 By counting the chromosome number of root tip cells in 18 regenerated plants derived from protoplasts of Actinidia eriantha Benth. ,the authors found 12 euploid plants and 6 mixoploid plants. Of the 12 euploid plants,6 were diploid (2n = 2x = 58) and the other 6 were tetraploid (2n = 4x = 116). The chromosome numbers of the mixoploid plants varied from 59 to 203. Multinucle-ate phenomenon was also observed in the interphase cells of 10 protoplast-derived plants. Cells with binuclei or trinuclei were common and cells having heptanuclei were also seen occassionally. Multin-ucleate phenomenon did not occur in the control, i. e. , the donor plant, where the chromosome number was 2n = 2x = 58.

  • 【文献出处】 Acta Botanica Sinica ,植物学报(英文版) , 编辑部邮箱 ,1997年02期
  • 【分类号】Q943
  • 【被引频次】18
  • 【下载频次】167
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