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水分胁迫对露花磷酸烯醇式丙酮酸羧化酶表达水平及特性的影响
The Effects of Water Stress on Leaf PEPCases in Mesembryanthemum cordifolium
【摘要】 水分胁迫能引起露花叶片PEP羧化酶的活力、酶蛋白和mRNA水平的提高。复水后,叶片PEP羧化酶表达量降低;茎中的PEP羧化酶在水分胁迫和恢复水分供应过程中变化情况与叶片相似,兼性CAM植物的碳代谢类型转变发生在植物的绿色组织中。露花叶片中除了250kD的PEP羧化酶同功酶外,还有300kD同功酶;主茎的叶片叶位越低,PEP羧化酶活力越高。
【Abstract】 Mesembryanthemum cordifoliu, is afacultative CAM plant. We found inthis plant that: 1. Water stress elevatedleaf PEPCase activity (Fig. 1 ), thecontent of PEPCase protein (Fig. 4 )and mRNA encoding PEPCase (Fig.5 ). 2. Rewatering lowered the leafPEPCase level (Fig. 1 ). 3. PEPCasesof stem and leaf were affected in thesame way either by water stress or byrewatering (Fig. 2 ). Therefore, it ispossible that the shifting from C3 toCAM occurred in all green parts of theplant when water stressed. 4. Therewas a 300 kD minor PEPCase isocnzyme besides the 250 kD major one(Fig. 2 ). They changed in the sameway during water stress and rewatering(Fig. 2). 5. The PEPCase activity inlower leaves was higher than that in theupper young leaves (Fig. 6).
【Key words】 phosphoenolpyruvate carboxylase; facultutive CAM plant; water stress; Mesembryanthemum;
- 【文献出处】 植物生理学报 ,ACTA PHOTOPHYSIOLOGICA SINICA , 编辑部邮箱 ,1997年02期
- 【分类号】Q945
- 【被引频次】28
- 【下载频次】238