【摘要】 ＰＣＲ法扩增出ＥＢ病毒ＤＮＡ酶全基因之后，用［γ－３２Ｐ］ＡＴＰ进行５’末端标记，然后利用凝胶迁移分析法（ｇｅｌｍｏｂｉｌｉｔｙｓｈｉｆｔａｓｓａｙ）来检测与该基因结合的蛋白质。结果表明在Ｒａｊｉ细胞核和细胞浆中均有ＥＢ病毒ＤＮＡ酶基因非特异性和特异性结合蛋白，在人乳清中也有与该基因结合的蛋白质。更多还原

【Abstract】 The EBV DNase gene was obtained by use of PCR. After the gene was labeled with [γ 32 P]ATP at the 5’ end, gel mobility shift assay was used to detect proteins binding to EBV DNase gene. In the nuclear extract and cytoplasm of Raji cells, there were proteins binding to the gene with both specificity and non specificity. In the human whey, there were also proteins binding to the gene, lactoferrin in which could not bind to it.更多还原