【摘要】 采用平板涂布的方法分离筛选到一种短藻丝体（２～３个螺旋）钝顶螺旋藻Ｓｐｉｒｕｌｉｎａｐｌａｔｅｎｓｉｓ藻株．该藻株在液体培养基中能生长成均匀分散的培养物，因而便于进行平板涂布、光密度测定以及定量取样等操作．进一步采用植物组织培养中常用的消毒试剂次氯酸钠处理藻丝体，获得了无细菌污染的纯培养物，可用于分子生物学和基因工程研究．另外，提供了一种成份比较简单易得的培养基，可用于钝顶螺旋藻的实验室常规培养更多还原

【Abstract】 Linear sweep voltammetry has been used to study the electrochemical behavior of transition metals structural units Ni(PPh 3) 2Cl 2 in DMF solution with 0 1 mol·L -1 Et 4NClO 4 on the Pt electrode. The results suggest that Ni(Ⅱ) is reduced to Ni(Ⅰ) at the first step and the process is reversible . The Ni(Ⅰ) is reduced to Ni(0) subsequently is an irreversible reaction. The Ni(Ⅰ) is not stable in solution and oxidated easily to Ni(Ⅱ) .更多还原