【摘要】 本文应用广泛性脑梗塞患者分离的、经免疫荧光与免疫酶染色证明为钩端螺旋体（简称钩体）L型的稳定株并以钩体原型为对照，感染豚鼠，感染次日两组动物体温均升高，感染原型组4／5从血液分离出钩体，L型组3／5分离出L型。2月后处死两组豚鼠取血分离，原型组钩体已从血中消失，而L型仍有3只分离出L型。病理切片证明两组病变主要为心、骨骼肌、肺、眼的间质性失，并有肝窦、小动脉狭窄，肺出血明显，脊髓前角神经细胞变性，原型组中有1只在肾曲小管中见有L型。结果表明，钩体L型稳定株可长期在血中存活能引起眼、神经后发症。原型感染后从血中迅速消失，但L型在肾中存在。说明原型在体内可转变为L型，并从尿中排出，成为一重要的传染源。更多还原

【Abstract】 Atrain of leptospira stable L-forms,isolated from patient with cerebral obstruction and identified with immunofluorescence and immunoenzyme technic was used for studing its pathogenicity in laboratory animals. Guinea -pigs infected with this L-forms were taken as the experimental group,and with leptospira original forms as the control group. On the 2nd day after infection,fever occurred in animals of both 2 groups and organisms wereisolated from blood in 3/5 of the experimental group and 4/5 of the control group. All animals were sacrificed 2 months after infection,and blood were taken again for cultivation,organisms could still be isolated from 3 of the experimental group,but none from the control group. In histological examination,the pathological changes found were mainly interstitial inflammations of eyes, heart,skeletal muscles and lungs,narrowing of sinuses and small arteries of liver,hemorrhages in lungs and degeneration of the nerve cells in the anterior horn of spinal cord in borh 2 groups. Leptospira L-forms were also seen in the convoluted tubules of 1 animal of the controll group.These results indicate that the stable L-forms might persist in the blood much longer than the original forms.And the late complication of the leptospirosis might be caused by L-forms induced through the medical treatment and body immunity in certain individuals. Leptospira L-forms could also be excreted in the urine and become a source of infection that should not be neglected.更多还原