【摘要】 应用逆转录病毒载体PLXSN将人的IL-2基因和抗新霉素基因(Neo~R)插入人肺腺癌细胞,转染以后,经G418筛选,获得阳性细胞.将转染与未转染细胞作比较,观察了两者在体外的生长特性.测量了阳性细胞上清液中IL-2含量.PCR证实了外源性基因的插入及持续稳定表达.另外在电镜下,可见转染细胞表面绒毛变短变少,此变化是否会影响细胞的浸润性及转移性尚待进一步研究.更多还原

【Abstract】 In this experiment, we introduced human interleukin-2(lL-2)gene and Neomycin resistance (Neo~R) gene into a human lung adenocarcinoma cell line GLC, using a retroviral vector PLXSN. The positive cells obtained by selecting in G418 were compared with the wild-type cells. We observed their growth characteristics in vitro and the quantity of IL-2 in the culture supernatants. PCR result demonstrated the integration of foreign genes into tumor genome DNA after transfection and permanent existence. Under electron microscope, what can be seen was the surface villi of trans-duced-cells are less and shorter. It deserves further investigation to determine whether the change of cell surface influence metastatic ability of cells.更多还原