【摘要】 已发现SAC-IIB₂淋巴瘤细胞株中原癌基因c-fos呈过度表达.针对小鼠c-fos cDNA第2-7编码序列,设计合成了18聚反义、正义和无义的寡脱氧核苷酸.结果发现,反义c-fos寡脱氧核苷酸能促使SAC-IIB₂的细胞形态向成熟阶段分化并抑制其增殖速率,同时降低其对裸鼠的致瘤性及Fos蛋白的表达,但对SAC-IIB₂的细胞动力学没有明显的影响.而正义和无义的c-fos寡脱氧核苷酸没有相应的作用.提示反义c-fos寡脱氧核苷酸能特异地抑制小鼠淋巴瘤SAC-IIB₂细胞的恶性表型.更多还原

【Abstract】 It was found that protooncogene c-fos was over-expressing in SAC-IIB₂ cell strains. Targeting the code 2-7 sequence of c-fos cDNA of mice, we designed and synthesised eighteen mers antisense. sense and nonsense oligodeoxynucleotides（ODN）. The results indicated that antisense c-fos ODN could induce SAC- IIB₂ maturative differentiation, inhibit its growth rate, decrease its ability of forming tumor in nude mice and expression of Fos protein, however, antisense ODN has no remarkable effect on cellular dynamics of SAC-IIB2. But sense and nonsense c-fos ODN have no similar effect on SAC-IIB₂ cell strains. The results demonstrated that antisense c-fos oligodeoxynucleotide has inhibition effects on malignant phenotype of mice SRS lymphoma cell.更多还原