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反义c-fos寡脱氧核苷酸对淋巴瘤细胞株SAC-IIB2恶性表型抑制的研究

The Study of Inhibition of Malignant Phenotype of SAC-IIB2 Cell Strains by Antisense Oligodeoxynucleotide of c-fos

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【作者】 殷连华孔宪寿严开平郑颂国

【Author】 Yan Kaiping, Yin Lianhua, Kong Xianshou et al (Department of Pathophysiology, Shanghai Medical University, Shanghai 200032)

【机构】 上海医科大学病理生理教研室上海市儿童医院上海医学遗传研究所上海医科大学肿瘤医院病理科 上海200032上海200032200040200032

【摘要】 已发现SAC-IIB2淋巴瘤细胞株中原癌基因c-fos呈过度表达.针对小鼠c-fos cDNA第2-7编码序列,设计合成了18聚反义、正义和无义的寡脱氧核苷酸.结果发现,反义c-fos寡脱氧核苷酸能促使SAC-IIB2的细胞形态向成熟阶段分化并抑制其增殖速率,同时降低其对裸鼠的致瘤性及Fos蛋白的表达,但对SAC-IIB2的细胞动力学没有明显的影响.而正义和无义的c-fos寡脱氧核苷酸没有相应的作用.提示反义c-fos寡脱氧核苷酸能特异地抑制小鼠淋巴瘤SAC-IIB2细胞的恶性表型.

【Abstract】 It was found that protooncogene c-fos was over-expressing in SAC-IIB2 cell strains. Targeting the code 2-7 sequence of c-fos cDNA of mice, we designed and synthesised eighteen mers antisense. sense and nonsense oligodeoxynucleotides(ODN). The results indicated that antisense c-fos ODN could induce SAC- IIB2 maturative differentiation, inhibit its growth rate, decrease its ability of forming tumor in nude mice and expression of Fos protein, however, antisense ODN has no remarkable effect on cellular dynamics of SAC-IIB2. But sense and nonsense c-fos ODN have no similar effect on SAC-IIB2 cell strains. The results demonstrated that antisense c-fos oligodeoxynucleotide has inhibition effects on malignant phenotype of mice SRS lymphoma cell.

【基金】 国家教委博士点(93011)基金资助
  • 【文献出处】 中国肿瘤生物治疗杂志 ,Chinese Journal of Cancer Biotherapy , 编辑部邮箱 ,1997年01期
  • 【分类号】R733.1
  • 【被引频次】6
  • 【下载频次】20
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