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胎盘中人绒毛膜促性腺激素的提取
Extraction of Human Chorionic Gonadotropin
【摘要】 本文报道由胎盘提取人绒毛膜促性腺激素的新方法。胎盘经过匀浆,分级分离,用弱酸性硅酸铝吸附,上柱用醋酸胺-乙醇溶液洗脱,乙醇沉淀,所得HCG粗品效价为400IU/mg,活性回收率达50%。
【Abstract】 This paper reports the method of preparing crude human ckorionic gonadotropin fromplacenta. The method was based on that the weakly acidic altrnminosidicate absorbed HCG of placenta.The .absorbent was washed with 40% ethanol containing 10% ammonium acetate solution into a column.The crude product posscsscd HCG potency of 400IU/mg and HCG recovery was 50%.
【关键词】 人绒毛膜促性腺激素;
胎盘;
弱酸性硅酸铝;
【Key words】 Human chorionic gonadotropin; Weakly acidic alumn~inosidicate; Placenta;
【Key words】 Human chorionic gonadotropin; Weakly acidic alumn~inosidicate; Placenta;
- 【文献出处】 镇江医学院学报 ,Journal of Zhenjiang Medical College , 编辑部邮箱 ,1997年04期
- 【分类号】R284
- 【被引频次】3
- 【下载频次】123