【摘要】 目的　为寻找新的肿瘤细胞诱导分化剂 ,探讨了淫羊藿甙 ( icariin,ICA)的诱导分化作用及其机制。方法　选用从朝鲜淫羊藿中提取的单体成分 ICA,采用四唑氮蓝 ( NBT)还原试验 ,12 5I-标记的环磷酸腺苷 ( c AMP)和环磷酸鸟苷 ( c GMP)双抗体分离技术以及扫描电镜技术 ,观察了 ICA对人急性早幼粒白血病细胞 ( HL- 60 )分化的影响。结果　ICA( 0 .1g/ L)作用后的 HL- 60细胞 ,NBT还原能力明显增强 ,细胞内 c AMP/ c GMP比值升高 ,细胞膜表面发生明显变化 ,出现较多的皱褶和球状突起。结论　ICA对 HL- 60细胞有诱导分化作用 ,其机制可能与升高细胞内 c AMP/ c GMP比值有关。更多还原

【Abstract】 Objective In order to search for new tumor differentiation inducer, the effects and mechanism of lcariin were studied. Methods Icariin is an monomer purified from Epimedium Koreanum Nakai. The differentiation of induction effects of lcariin were observed on the human promyelocytic leukemic cell (HL 60) by NBT reduction test, 125 I cAMP and 125 I cGMP double antibody radioimmune methods, scanning electronic microscopy technique. Results After treating HL 60 cells with lcariin at concentration 0.1g/L, reduction of NBT and cAMP/cGMP ratio were increased. There were many rugosities and ball like processes on the cell surface. Conclusion lcariin had the effects of induction of differentiation on HL 60 cells, the mechanism might be related to elevating the cAMP/cGMP ratio.更多还原