【摘要】 目的　探讨白细胞介素 - 6( interleukin- 6,IL- 6)对人肺癌细胞体外生长的影响。方法　应用逆转录 PCR技术检测人肺腺癌细胞系 PAa IL- 6受体 m RNA、Northern杂交技术检测 PAa细胞IL- 6m RNA;用生物活性检测法测定 PAa细胞所分泌的 IL- 6,并用抗体中和法检测内源性 IL- 6对PAa细胞体外生长的影响。结果　PAa细胞表达 IL- 6受体 m RNA,重组人 IL- 6刺激 PAa细胞生长 ;PAa细胞表达 IL- 6m RNA且分泌有生物活性的 IL- 6,抗 IL- 6抗体抑制 PAa细胞生长。结论　 IL- 6对 PAa细胞生长具有调控作用 ,IL- 6为 PAa细胞自分泌生长刺激因子。更多还原

【Abstract】 Objective To study the effect of interleukin 6(IL 6) on the growth of a human lung adenocarcinoma cell line PAa in vitro. Methods IL 6 receptor (IL 6R) mRNA level was detected by means of reverse transcription polymerase chain reaction (RT PCR) and IL 6 mRNA was analyzed using Northern blotting hybridization. The production of IL 6 was detected by bioassay. The biological effect of IL 6 released by PAa cells was assessed with anti IL 6 neutralizing antibodies. Results PAa cells expressed IL 6R mRNA. Recombinant human IL 6 stimulated the proliferation of PAa cells. PAa cells also expressed IL 6 mRNA and produced bioactive IL 6. Treatment of PAa cells with anti IL 6 antibodies resulted in decreased growth of PAa cells. Conclusion These results demonstrate that IL 6 acts as an autocrine growth stimulator for PAa cells in vitro.更多还原