【摘要】 为观察８株鼻咽癌细胞株（ＣＮＥ１、ＣＮＥ２、ＨＮＥ１、ＨＮＥ２、ＨＮＥ３、ＨＯＮＥ１、ＳＵＮＥ１和ＨＫ１）Ｒｂ基因的结构和表达情况。在Ｓｏｕｔｈｅｒｎ杂交，除ＨＫ１出现一微弱的额外条带外，其余７株未见异常。聚合酶链反应分析发现ＣＮＥ１和ＳＵＮＥ１的Ｒｂ基因外显子１０和ＨＯＮＥ１的外显子１３扩增阴性。免疫沉淀／免疫印迹可见８株鼻咽癌细胞株均出现分子量正常的Ｒｂ蛋白条带。免疫组织化学则见生长活跃的细胞中强阳性反应细胞数远较静止和衰老的细胞为多。综上所述，８株鼻咽癌细胞株中４株可能有微小的Ｒｂ基因变化。免疫组化强阳性可能表示８株鼻咽癌细胞株部分细胞Ｒｂ基因表达异常。更多还原

【Abstract】 This investigation sought to address if there is any change of Rb gene in 8 nasopharyngeal carcinoma (NPC) cell lines and, if yes, to observe the differences among them. Using Southern hybridization method, we observed normal hybridization patterns in all these cell lines except HK1 which showed a weak extra band, PCR analyses discovered negative amplification of Rb gene exon 10 in CNE1 and SUNE1 and exon 13 in HONE1. In immunpoercipitation/immunoblotting assays, normal sized RB protein band appeared in all 8 NPC cell lines. By means of immunohistochemical technique, much more cells with strong positive reaction (overexpression) were observed in the active growing region, compared with those observed in the static or aging region. To put the above results together, 4 out of 8 NPC cell lines may have some subtle changes in Rb gene, The strong positive reaction in a portion of cells in these 8 NPC cell lines may represent some abnormal (over) expression of the gene.更多还原