【摘要】 目的 建立一个在胸腺内表达外源性主要组织相容性抗原复合体(MHC)抗原的实验模型,为下一步研究作准备。方法 应用逆转录病毒载体介导的基因转移技术,首次将外源性MHC基因转移到T淋巴表达并应用聚合酶链反应(PCR)及反转录聚合酶链反应(RT-PCR)检测转染T细胞DNA及mRNA。结果 外源性MHC基因已整合到靶细胞染色体DNA并有效地转录;单克隆抗体免疫荧光染色流式细胞仪检测显示在转染T细胞膜有外源性MHC分子表达;转染效率为46.2％。结论 外源性MHC基因可以转移到T细胞稳定表达,为今后的研究提供了经验。更多还原

【Abstract】 Objective To establish a model expressing allogenic major histocompatibility complex (MHO gene in thy-mus for further study. Methods Allogenic MHC gene was transferred into mice T lymphocytes by retroviral-me-diated gene transfer technique. After gene transfer 72 hours, DNA and RNA were prepared from transfected T cells for PCR and RT-PCR analysis. Results Allogenic MHC gene has integrated into the chromosomal DNA of the T cells and was effectively transcripted in the transfected T cells. Flow cytometry assay showed that allogenic MHC molecules were expressed on the membrane of transfected T cell after transfection 72 hours and that trans-feet efficiency was 46. 2% < using anti-mouse MHC monoclonal antibodies by immunofluorescence stain. Conclusion Allogenic MHC gene can be transferred into T cells and be stably expressed in T lymphocytes.更多还原