【摘要】 目的:研究静脉移植术后内膜增生(IH)中细胞基质(ECM)堆积的发生、发展及与平滑肌细胞(SMC)增殖的关系.方法:将大鼠一段颈外静脉桥接移植入颈总动脉,运用组织切片的特殊染色及免疫组化方法,观察术后1天至24周移植静脉的IH中,Ⅰ型胶原(ColⅠ)、Ⅱ型胶原(ColⅢ型)及SMC结蛋白(D_m)的变化.结果及结论:术后2周,IH中开始出现胶原的堆积,术后12周,IH中胶原含量达最大值并自此保持稳定不变,术后24周IH主要由细胞外基质组成;术后2周,IH中SMC的D_m开始表达,表明SMC的表型开始由收缩型向分泌型改变,术后6周,D_m达最大值,表明此时分泌型SMC最多,SMC可能是分泌细胞外基质的间质效应细胞.更多还原

【Abstract】 The autogenous jugular vein was grafted into the carotid artery in a rat. The grafts were haversted at 1,2,4,7 days and 2,4,6.12,18,24 weeks after implantation. The histological cross section of each specimen was stained with van gieson and weigert stain for morphometric evaluation. The smooth muscle cell (SMC) phenotypic modulation and collagen I (col I) and collagen III (col III) expression in the neointima of vein graft were detected by immunohis-tochemical methods with monospecific antibodies a-gainst Desmin (Dm), col I , col III . The experimental results showed: 1) The phenotype of SMC in the neointima revealed a transformation from the contractile into the synthetic 2 weeks after grafting. Synthetic phenotype SMC were highest at 6 weeks after grafting. 2) Col I appeared in the neointima 2 weeks and reached peak 12 weeks after grafting. Then it remained steady. 3) col I in the neointima appeared earlier and increased more quickly than col III .更多还原