【摘要】 目的:探索促进肌腱细胞增殖的方法。方法:将原代人死胎肌腱细胞和第6代、第13代冻存后复苏的人胎儿肌腱细胞进行体外培养,并在培养基中加入不同浓度的类胰岛素生长因子₁（insulin-likegrowth factor 1,IGF-₁）。通过绘制生长曲线及³H-TdR渗入等方法,观察IGF-₁对肌腱细胞的作用。结果:IGF-₁能使培养的肌腱细胞增殖加快,提前进入平顶期。肌腱细胞的增殖与IGF-₁的浓度在一定范围内呈现量效关系。同时IGF-₁的作用并不导致肌腱细胞的肥大。结论:IGF-₁对人胚胎肌腱细胞增殖具有明显的促进作用。更多还原

【Abstract】 Objective:To investigate whether insulin-like growth factor₁(IGF_-1) induces the proliferation of human fetal tenocyte. Methods:The primary tenocytes of dead human fetus and the reanimated 6th and 13th generation tenocytes were incubated with IGF_-1 at different concentrations. The influence of IGF_-1 on tenoeyte was observed by means of growth curve and 3^H-TdR permeation. Results:The tenocyte incubated with IGF_-1 at the concentration of 5μg/L reached the stagnate phase one day earlier than the control,There was dose-effect relationship between IGF_-1 concentration and tenocyte proliferation at concentration of 1.25μg/L to 40μg/L. Tenocyte proliferation increased in a dose dependent fashion after incubation with IGF_-1. Furthermore,IGF_-1 stimulated hyperplasia but not hypertrophy in this in vitro system. Conclusion: IGF_-1 has an obvious effect in promoting human fetal tenocyte proliferation.更多还原