【摘要】 将絮凝技术应用于Ｄ－葡萄糖直接串联发酵产生维生素Ｃ前体——２－氧代－Ｌ－古洛糖酸（１）这一新工艺中第二步菌株（即能将中间体２，５－二氧代－Ｄ－葡萄糖酸转化为１的棒状杆菌ＳＣＢ３０５８）发酵液的处理。系统研究了絮凝处理的最适条件。实验结果表明，絮凝剂的种类和浓度、处理过程中的ｐＨ值和温度，是影响絮凝效果的关键因素。将发酵液用适当的絮凝剂在浓度２０００ｐｐｍ、ｐＨ７．００和温度４０°Ｃ的条件下处理４ｈ所除去的固体杂质比用离心法多４．２５倍。更多还原

【Abstract】 The flocculation technology was used to treat fermentation broth of Corynebacterium sp.SCB 3058 which was able to convert 2,5 diketo D gluconate,an intermediate of D glucose tandem fermentation,to 2 keto L gulonic acid,the precursor of vitamin C.Optimum conditions of flocculation were systematically studied.Experimental results showed that the type and concentration of flocculant,the pH value of treated broth,and the temperature of treatment process affected the flocculation greatly.When the broth was treated with suitable flocculant(concentration 2000 ppm) at pH7.00,40°C for 4 h,the removed solid precipitate was 5.25 times as many as those obtained from the centrifugation method.更多还原