【摘要】 目的观察人胚肺二倍体成纤维细胞的老年细胞中原癌基因ｃ－ｆｏｓ、ｃ－ｍｙｃ表达的变化及其与特异转录因子的关系。方法使用Ｎｏｒｔｈｅｒｎ印迹杂交法检测ｃ－ｆｏｓ、ｃ－ｍｙｃ基因表达情况，使用Ｓｏｕｔｈｗｅｓｔｅｒｎ印迹杂交法检测特异转录因子。结果表皮生长因子（ＥＧＦ）对老年细胞中ｃ－ｆｏｓ、ｃ－ｍｙｃ转录的可诱导性下降，使与ｃ－ｆｏｓ基因片段特异结合的蛋白质Ｐ９１和与ｃ－ｍｙｃ基因片段特异结合的蛋白质Ｐ８０明显降低。结论老年细胞中ｃ－ｆｏｓ、ｃ－ｍｙｃ基因转录对ＥＧＦ可诱导性下降，可能与其结合的转录因子活性改变有关。更多还原

【Abstract】 Objective To observe the changes of proto oncogene c fos/c myc expression and its relation to specific transcription factors in human senescent fibroblast after epidermal growth factor (EGF) addition. Methods The c fos/c myc expression were analysed by Northern blot. Transcription factors were analysed by Southwestern blot. Results (1)The expression of c fos/c myc was less susceptible to induction by EGF as the cells aged.(2)EGF increased the binding of protein P91 to c fos with regulatory sequence and P80 to c myc with regulatory sequence, but the increased levels of both proteins were significantly reduced in senescent cells. Conclusions Inability of c fos/c myc gene induction by EGF in senescent cells might be correlated with some DNA binding proteins.更多还原