【摘要】 目的了解 HCV 感染后细胞免疫在其中所起作用.方法对27例慢性丙肝患者及15例正常献血员,以 MTT 法检测慢性丙肝患者外周血单核细胞(PBMC)对 HCV 不同区抗原的增殖反应。结果与正常人相比,慢性丙肝患者PBMC 对 HCV 抗原 C22、C33有明显增殖反应,(P值<0.01)而对 NS5却无明显增值。C22抗原性最强。结论在慢性 HCV感染时可同时存在针对 HCV 相关抗原的细胞免疫和体液免疫,这种细胞免疫不能消除 HCV 感染,并且疾病的状态与这种细胞免疫无关。更多还原

【Abstract】 Objective To understand the role of cell mediated immunity in HCV infection.Methods Twenty-seven patients with chronic hepatitis C and fifteen normal health subjects were studied for their peripheral blood lymphomononuclear cell(PBMC)proliferative responses to hepatitis C virus structural and nonstructural antigens C22(core),C33(NS3),NS5 by the method of MTT.Results PBMC from chronic hepatits C patients sigenificantly proliferated in vitro in response to the HCV antigen C22,C33 by comparison with that of normal subjects,P<0.01. But there was no sigenificant proliferative response to NS5 Ag.This showing that it is true that there are cellular immune to HCV antigens in peripheral blood in patients with chronic Hepatitis C.and core Ag is the most immunogentic HCV Ag.We also found that this cellular immune response did not correlate with humoral immune responses,and have no significant increase in patients with HCV RNA in peripheral blood cell turning to negative. Conclusions Sdudies showed that there were the cellular and humoral immune responses to HCV-related antigen. Disease status did not correlate with the peripheral lymphocyte immune response.更多还原