【摘要】 为探讨莫尼地平对新生儿缺氧缺血性脑病的防治作用，将８６只新生猪分为正常对照组，缺氧缺血性脑损伤（ＨＩＥ）１小时（１ｈｒ）、２４ｈｒ、７２ｈｒ组，尼莫地平０．５μｇｋｇ－１ｍｉｎ－１治疗后１ｈｒ、２４ｈｒ、７２ｈｒ组，尼莫地平１μｇｋｇ－１ｍｉｎ－１治疗后１ｈｒ、２４ｈｒ、７２ｈｒ组。检测各组红细胞胞浆总钙（ＲＢＣＴＣａ）及游离钙（ＲＢＣＣａ２＋ｉ）的浓度变化。结果：ＨＩＥ１ｈｒ组ＲＢＣＴＣａ和Ｃａ２＋ｉ均明显高于正常组对照（Ｐ＜０．０１），随着缺氧缺血状态的改善，ＲＢＣＴＣａ和Ｃａ２＋ｉ含量逐渐下降，ＨＩＥ７２ｈｒ组接近正常对照组（Ｐ＞０．０５）。两种剂量尼莫地平各治疗组ＲＢＣＴＣａ和Ｃａ２＋ｉ均明显低于ＨＩＥ１ｈｒ和２４ｈｒ组（Ｐ＜０．０１），与正常对照组比较，差异无显著意义（Ｐ＞０．０５）。提示：窒息后细胞内钙的沉积与新生猪ＨＩＥ的发生有关，尼莫地平能阻止Ｃａ２＋内流，有效地保护神经细胞。更多还原

【Abstract】 The objective of this study was to investigate the changes in total calcium (TCa) and free calcium iron (Ca 2+ i) concentration in RBC cytoplasm in piglets and to study the pathogenesis of hypoxic ischemic encephalopathy (HIE) and protective effect of two different doses of nimodipine on HIE. TCa and Ca 2+ i of RBC were measured in 86 piglets which were divided into the following groups: normal control group ( n =9), post HIE groups [1 hour ( n =8), 24 hours ( n =8) and 72 hours ( n =8)], nimodipine (1 μg·kg -1 ·min -1 ) treatment groups [1 hour ( n =10), 24 hours ( n =10) and 72 hours ( n =9)] and nimodipine (0.5 μg·kg -1 ·min -1 ) treatment groups [1 hour ( n =8), 24 hours ( n =8) and 72 hours ( n =8)]. The results showed that RBC TCa and Ca 2+ i concentrations were significantly higher in HIE model 1 hour group than those in normal control group ( P <0.01) and then gradually reduced with time and approached to a level of the normal control group after 72 hours of HIE ( P >0.05). RBC TCa and Ca 2+ i were evidently lower in both different doses of nimodipine treatment groups than those in post HIE group ( P <0.01) and approached to a level of the normal control group ( P > 0.05 ). The results suggest that postasphyxiated intracellular calcium accumulation is associated with newborn HIE and that the calcium antagonist, nimodipine, is effective in protecting brain cells from further damage through its influence on cerebral calcium accumulation.更多还原