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竞争性逆转录聚合酶链反应定量检测对HCV RNA
Quantive detection of HCV RNA by competitive RT PCR
【摘要】 为了解感染丙型肝炎病素(HCV)后的(HCVRNA)含量与丙型肝炎病情轻重,预后,治疗反应的关系,采用竞争性逆转录聚合酶链反应(CRT-PCR)的方法,对9例感染了HCV患者的血清进行了HCVRNA的定量检测。结果:6.6×103拷贝1毫升占88.9%,6.6×104拷贝/毫升占11.1%。结果提示:急慢性丙型肝炎及无症状HCV感染者体内均含有较高的病毒滴度,且滴度低预后好,抗病毒疗效好。
【Abstract】 In order to determine the relationship between the amount of HCV RNA in sera from patients with HCV infection and severity of live disease prognosis and reations to therapy, HCV RNA was detected quantitatively in the sera from nine patients infected with HCV by competitive RT PCR. The results showed that the 6.6×10 3 copies of HCV RNA were found in 88.9% of these petients and 6.6×10 4/ml in 11.1%, respectively. This suggested that there was high tiler of HCV in both acute and chronic patients with viral hepatitis and asymptomic HCV carriers, and these patients with low titer of HCV RNA had good prognosis and reation to anti viral therapy.
- 【文献出处】 中华传染病杂志 ,CHINESE JOURNAL OF INFECTIOUS DISEASES , 编辑部邮箱 ,1997年01期
- 【分类号】Q78
- 【被引频次】2
- 【下载频次】22