【摘要】 目的观察碱性成纤维细胞生长因子（ｂＦＧＦ）的自分泌现象及其与反应性星形胶质化的关系。方法在大鼠星形胶质细胞（ＡＳ）原代培养的机械损伤模型上，以免疫细胞化学及原位杂交技术检测ｂＦＧＦ、ＰＣＮＡ、ＧＦＡＰ和ＧＦＡＰ－ｍＲＮＡ的动态变化。结果（１）损伤边缘的ＡＳ于伤后２小时起表达ｂＦＧＦ，１２小时达高峰，２天后回落；（２）损伤边缘的ＡＳ于伤后６小时起表达ＧＦＡＰ－ｍＲＮＡ，１天达高峰，２天后回落；（３）伤后１天起，ＧＦＡＰ表达明显增强，ＡＳ胞体肥大并向损伤区伸出粗大突起，２天时ＧＦＡＰ表达至高峰；（４）损伤边缘的部分ＡＳ从伤后２小时起表达ＰＣＮＡ，１天时达到高峰。结论（１）受损的ＡＳ能自分泌ｂＦＧＦ，这是ＡＳ受损后的早期反应之一，具有促进反应性星形胶质化的作用；（２）反应性星形胶质化时，ＧＦＡＰ表达增强是转录水平上ＧＦＡＰ－ｍＲＮＡ增加的结果；（３）反应性星形胶质化以ＡＳ肥大为主，增生为辅。更多还原

【Abstract】 ObjectiveTostudytherelationshipbetweenreactiveastrogliosisanditsau┐tocrinebFGF.MethodsOntheprimaryastrocyteculturewithmechanicalscratch,thedynamicexpres-sionofbFGF,PCNA,GFAPandGFAP-mRNAweredeterminedbyimmunocytochemistryandinsituhybridization.ResultAstrocytesattheedgeoftheinjurybegantoexpressbFGF2hoursafterscratchingandreacheditspeakbythe12thhour,thendeclinedafter2days.GFAP-mRNAwasde-tectableinastrocytesneartheinjuryfromthe6thhourandreacheditspeakwithin24hours.Afewhy-pertrophicastrocytesintheinjuredareaexpressedGFAP-mRNAonthe3rdday.EnhancedGFAPex-pressionofastrocyteswasobservedwithhypertrophyofcytoplasmawhichextendedwideprocessesintotheinjuredareafromthedayofthescratchandreacheditspeakonthe2ndday.Onthe3rdday,thepre-viouslyinjuredareawascoveredwithhypertrophicastrocytes.PCNAwasexpressed2hoursafterdam-agebysomeoftheastrocytesinthevicinityofinjury.ConclusionAutocrinebFGFwastheearlyre-sponseofreactiveastrocytesandbFGFmayactaspromoterofreativeastrogliosis.EnhancedGFAPex-pressionwastheresultofupregulationofGFAP-mRNA.Theprominenteventofreativeastrogliosiswasthehypertrophyofastrocytes.更多还原