【摘要】 研究了¹⁵³Sm-EDTMP内照射治疗时对骨瘤细胞诱发的细胞凋亡过程。通过荧光显微镜观察,发现当骨瘤细胞受¹⁵³Sm-EDTMP内照射作用时,可诱发明显的核断裂和核固缩,以及凋亡小体形成为特征的细胞凋亡发生。微观放射自显影研究表明,¹⁵³Sm-EDTMP可掺入通过骨瘤细胞膜,在细胞内呈亲膜性积聚,也可被骨瘤细胞吞噬,在细胞质及细胞核内以吞噬体的形式滞留,并在膜包裹着的凋亡小体周围呈现。同时观察到,随着¹⁵³Sm-EDTMP内照射时间的延续,骨瘤细胞的增殖抑制率亦逐渐增升。更多还原

【Abstract】 The apoptosis of bone tumor cells treated with internal irradiation by 153Sm-EDTMP was studied. The morphological changes in bone tumor cells were observed by fluorescence microscopic and microautoradiographic observations. It was found that bone tumor cells internally irradiated with 153Sm-EDTMP, displayed significant nuclear fragmentation and marked pyknosis as well as apoptotic bodies formation. The microautoradiographic study showed that l53Sm-EDTMP could permeate through cell membrane and displayed membrane-seeking condensation in tumor cells. Soon afterwards 153Sm-EDTMP could be phagocytized by the tumor cells and distributed in cytoplasm and nucleus in the form of phagosome. With the prolongation of observing time , the membrane-bounded apoptotic bodies was observed. With the lengthening of internal irradiation time by 153Sm-EDTMP, the inhibition rate of proliferation of bone tumor cells increased progressively.更多还原