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薄层扫描色谱法测定洗剂中苯甲酸苄酯与邻苯二甲酸二丁酯的含量

TLC densitometric determination of benzylis benzoatis and dibulylis phthalatis in lotion

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【作者】 何娅焦正郁韫超

【Author】 He Ya, Jiao Zheng, Yu Yunchao (Huashan Hospital, Shanghai Medical University,Shanghai, 200040)

【机构】 上海医科大学华山医院

【摘要】 采用双波长薄层色谱扫描法同时测定复方苯甲酸苄酯洗剂中苯甲酸苄酯与邻苯二甲酸二丁酯的含量,点样后能将本品中两个有效成分很好地分离,在GF254板上,经甲苯∶二氯甲烷(2∶1)混合溶剂展开,反射锯齿扫描测定,λs235nm,λR260nm,以外标两点法定量。苯甲酸苄酯与苯二甲酸二丁酯测定的线性范围分别为6.0~30.0μg和10.0~50.0μg。方法可行,结果满意。

【Abstract】 Benzylis benzoatis (BB) and dibulylis phthalatis (DBP) in lotion were determined by dualwavelength TLC after the sample being spotted on a GF 254 silica gel plate, developed with a solvent system of toluene dichloromethane(2∶1) mixture. They were analyzed by densitometry at 235nm and 260nm. The peak area and samples concentration was calculated by external two point method and showed a linear relation in 6.0  ̄ 30.0 μg for BB and in 10.0  ̄ 50.0 μg for DBP. The results show that this method is reliable and satisfied.

  • 【文献出处】 中国医院药学杂志 ,CHINESE JOURNAL OF HOSPITAL PHARMACY , 编辑部邮箱 ,1997年07期
  • 【分类号】O657.7
  • 【被引频次】3
  • 【下载频次】75
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