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鬼针草染色体的检测与分析

Analysis of Bidens bipinnata chromosome

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【作者】 王冰徐泽红郑太坤徐兴家

【Author】 Wang Bing (Wang B), Xu Zehong (Xu ZH), Zheng Taikun (Zheng TK), et al (Department of Chinese Pharmacy, Liaoning College of Traditional Chinese Medicine, Shenyang 110032)

【机构】 辽宁中医学院中药系

【摘要】 目的:对鬼针草染色体核型等进行系统研究,为该种鉴定、演化和良种选育等提供必要的细胞学资料。方法:采用常规制片方法,结合显微摄影对染色体进行检测分析。结果:除观察到正常的2n=72外,还发现有2n=70的非整倍体。核型公式是K(2n)=6X=72=36m+28sm+6st+2t,染色体相对长度组成为2n=72=8L+26M2+34M1+4S,属于“2B”型。全组染色体总长是83.41μm,长臂总长53.62μm,核型不对称系数为62.43%,染色体总体积97.17μm3。结论:鬼针草染色体的数量、形态清晰,可为进一步的深入研究打下基础

【Abstract】 OBJECTIVE: To systematically study the evolution and fine seeds selection through Bidens bipinnata chromosome number, karyotype and volume. METHODS: Sections combined with micrograph were used to analyse chromosome karyotype etc. RESULTS: Except normal diploid 2n=72, aneuploid 2n=70 was found in the same individual plant root tip. Karyotype formula was based on Levan’s publication in 1964, K(2n)=6X=72=36m+28sm+6st+2t. According to the method of Kuo SR, the species chromosome based on the relative length was 2n=72=8L+26M 2+34M 1+4S, which belonged to "2B" type according to the Stebbings karyotype classification. The total length of chromosome groups was 83.41 μm, the total length of long arms 53.62 μm, and from Arano’s method, the AS.K% was 62.43% . The total volume of chromosome was 97.17 μm 3 according to De vescovi et al. CONCLUSION: Biden bipinnata chromosome number, morphological character are clear.

  • 【文献出处】 中国药学杂志 ,CHINESE PHARMACEUTICAL JOURNAL , 编辑部邮箱 ,1997年07期
  • 【分类号】R284.1
  • 【被引频次】6
  • 【下载频次】105
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