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新抗生素M-90产生菌原生质体制备、再生及对产抗生素的影响

Protoplast Formation and Regeneration from the Micromonospora M 90 and the Effects on Antibiotics Yield

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【作者】 王耀伟陈真余晓捷

【Author】 Wang Yaowei, Chen Zhen, Yu Xiaojie Department of Microbial Pharmaceutics, China Pharmaceutical University, Nanjing 210009

【机构】 中国药科大学微生物制药研究室

【摘要】 报道了新抗生素M-90产生菌原生质体的制备、再生及对产抗生素的影响。利用二级菌丝培养,在35℃、溶菌酶浓度为2mg/ml的条件下,酶解菌丝,形成原生质体达1011/ml;选择适当的再生条件,再生率达20%;原生质体再生株的抗生素产量比对照株提高34%。

【Abstract】 The paper reports the formation and regeneration of protoplast from the new antibiotics, Micromonospora M 90, as well as the effects on antibiotics yield producing strain. Under the conditions of 35℃ and 2 mg/ml of lysozyme, the secondary mycelia of M 90 was cultured and enzymelysed, and was protoplast formed at 10 11 /ml; the regeneration rate reached to 20% under the selected regenera ting conditions; the antibiotics yield of the regenerated protoplast colony was increased by 34% as compared with the control.

【关键词】 小单孢菌原生质体再生
【Key words】 MicromonosporaProtoplastRegeneration
  • 【文献出处】 中国药科大学学报 ,JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY , 编辑部邮箱 ,1997年02期
  • 【分类号】R978.1
  • 【被引频次】4
  • 【下载频次】51
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