【摘要】 为建立一种诊断和预测胰岛素依赖型糖尿病（ＩＤＤＭ）的免疫学定量检测指标，改良了Ｐｅ－ｔｅｒｓｅｎ的谷氨酸脱羧酶自身抗体（ＧＡＤ－Ａｂ）放射配体分析法，以试管内转录和翻译的标记６５ｋＤ谷氨酸脱羧酶作抗原，以蛋白Ａ凝胶作为抗体沉淀剂，用液闪仪测ｃｐｍ值。对１５例初发ＩＤＤＭ和４０例对照的测定结果显示：ＩＤＤＭ组ｃｐｍ值显著高于对照组（Ｐ＜０．００１）；以对照组ｘ±２ｓ作为阳性标准，则ＩＤＤＭ组阳性率为６７％（１０／１５），对照组为２．５％（１／４０）。故改良的ＧＡＤ－Ａｂ放射配体分析法对初发ＩＤＤＭ诊断的特异性为９７．５％，敏感性为６７％。且操作简便，易于推广。更多还原

【Abstract】 To establish a quantitative assay of immunological marker(GAD Ab) for type Ⅰ diabetes(IDDM), Petersen’s radioligand assay(RLA) of GAD Ab was modified as follows:In vitro transcribed and translated GAD 65 was used as a tracer, Protein A sepharose was used as a precipitator of antibody, and the liquid scintillation counter was used to measure cpm value. The assay results of GAD Ab in 15 recent onset IDDM and 40 healthy controls showed that the cpm values of IDDM group were higher than that of the control group(P<0 001); In 10 of 15 (67%) IDDM patients and 1 of 40 controls cpm values were over the positive standard(control group cpm mean values +2s), so that the sensitivity and specificity of RLA for GAD Ab in IDDM were 67% and 97.5%; the method was simple and easy to be applied.更多还原